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49 CFR Part 40, "Procedures for Transportation Workplace Drug and Alcohol Testing Programs," tells employers and service agents in every DOT agency how to conduct drug and alcohol tests. Each topic below reproduces a subpart or appendix; the official current text is on eCFR.

Program and employer rules

• Subpart A: Administrative provisions: who the rule covers and definitions.
• Subpart B: Employer responsibilities: the DER, previous employer checks, and stand-down rules.
• Part 40 final rule (2023): summary of changes: oral fluid testing and other 2023 amendments.

Drug testing

• Subpart C: Urine collection personnel and Subpart D: Collection sites, forms, and supplies
• Subpart E: Urine specimen collections
• Subpart F: Drug testing laboratories
• Subpart G: Medical Review Officers and verification
• Subpart H: Split specimen tests and Subpart I: Problems in drug tests, including refusals

Alcohol testing

• Subpart J: Alcohol testing personnel and Subpart K: Testing sites, forms, and equipment
• Subpart L: Alcohol screening tests and Subpart M: Alcohol confirmation tests
• Subpart N: Problems in alcohol testing, including refusals

After a violation, privacy, and service agents

• Subpart O: SAPs and the return-to-duty process
• Subpart P: Confidentiality and release of information
• Subpart Q: Service agents and Subpart R: Public interest exclusions

Appendices

• Appendix A: Urine collection kit standards
• Appendix B and Appendix C: semi-annual laboratory reports
• Appendix D: Split specimen failure to reconfirm
• Appendix E: SAP equivalency requirements
• Appendix F: What C/TPAs may transmit to employers
• Appendix G: Alcohol testing form
• Appendix H: MIS data collection form