Last Updated: 03/13/2019

DOT Rules and Regulations / How Can I Become a Collector For DOT Drug Testing?

How do I become qualified to be a collector?

To become qualified as a collector, you must be knowledgeable about Part 40 regulations, the current “DOT Urine Specimen Collection Procedures Guidelines,” and DOT agency regulations applicable to the employers for whom you will perform collections. You must also stay current on any changes to these materials. Additionally, you must (1) successfully complete a qualification training program and (2) pass a monitored proficiency demonstration, as required by DOT regulations [See 49 CFR Part 40.33 (b–c), effective August 1, 2001].

Note: There is no “grandfather” clause or waiver for this requirement.
A collector’s qualifications are not location- or collection-site-specific, and eligibility follows them anywhere DOT agency-regulated urine specimens are collected.

There is no requirement for qualified collectors to register with or be listed on any federally maintained or federally sponsored registry. However, they are required to maintain documentation (for federal inspection) of successful completion of their training and proficiency demonstration.

Do I need to become “certified” to conduct DOT urine collections or operate a DOT collection site for drug testing?

No, the DOT does not require “certification” of a collector or a collection site to perform DOT urine collections. Only individual collectors (not collection sites) may become qualified.

What should be the content of the qualification training program, and who may conduct it?

Although the DOT has not published a set curriculum for urine collection training, trainers are expected to base their programs on DOT regulations and the latest version of the “Urine Specimen Collection Guidelines.” (See the final question in this document for access instructions.)

The qualification training [See 40.33(b)] must include:
Knowledge of Part 40 collection procedures

  • All steps necessary to conduct a proper collection
  • How to complete the Custody and Control Form (CCF)
  • How to handle problem collections (e.g., shy bladder, tampering)
  • Understanding of fatal and correctable flaws in collections and paperwork
  • The collector’s responsibility to maintain the integrity, privacy, and security of the process


Anyone may conduct the qualification training. The trainer does not need to be an experienced collector or even a person (e.g., an internet course is acceptable). Training may be delivered in person, by video, computer programs, the internet, video conferencing, or other equivalent means. There is no minimum time requirement for the training.

Although an exam is not required, it is recommended that training include a method to verify comprehension.

What should be the content of the collector proficiency demonstration and who can conduct it?

After completing the training, the collector must perform five error-free mock collections under direct observation by a qualified monitor [See 40.33(c)].

These mock collections must include:

  • 2 uneventful/routine
  • 1 for insufficient urine quantity
  • 1 for temperature out of range
  • 1 for refusal to sign the CCF or initial the seal

Mock collections must be monitored in real time, either in person or via a live video link with direct interaction. A checklist is acceptable if it was part of the training and will be used in real collections.

The monitor must be a qualified collector who:

  • Has performed DOT collections for at least one year, or
  • Has provided Part 40 training for one year, or
  • Has completed a train-the-trainer program

This person must also provide written attestation that all mock collections were error-free [See 40.33(c)(2)].

What kind of documentation must I keep to show that I’m qualified?

Most organizations provide a certificate upon successful course completion. Collectors must be prepared to demonstrate eligibility to DOT inspectors, employers, and service agents (e.g., C/TPAs) [See 40.33(g)].

You should also be ready to provide detailed descriptions of the training and mock collections, such as certificates or signed letters from trainers or observers confirming completion.

Do I have to go through refresher training?

Yes. Qualified collectors must complete refresher training every five (5) years to remain eligible [See 40.33(e)]. The content must be the same as or equivalent to the initial training and mock collection. Collectors are expected to stay updated with any regulatory changes, not wait for refresher training.

Does DOT offer collector training?

No. The DOT does not offer training, maintain lists of programs or trainers, nor certify or endorse any organization’s training.

Collection sites may either conduct their own training or hire an external provider. You can find such services through online searches, industry associations, or training directories.

Once I’m qualified, how do I get business?

Becoming a collector is a business decision. You are responsible for growing your business. Consider transportation trade shows, local directories, or online platforms to find clients regulated by DOT.

Where can I get more information?

Visit the DOT Office of Drug & Alcohol Policy & Compliance (ODAPC) at www.dot.gov/ost/dapc. From there, navigate to the “Urine Collection Personnel” page to download the latest Part 40 and collection guidelines.

If you don’t have web access, call 202-366-3784 to request a hard copy.

Related Topics:

Do you need more information?

If you need our assistance, or want to know more about Med-Stop, please use our Support contact form, send e-mail to info@med-stop.com or call at 1-(877) 633-3633

I_Please describe Your problem, question.