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How Can I Become A Collector For Dot Drug Testing?
Last Updated: 07/07/2026

To become qualified as a collector, you must be knowledgeable about Part 40 regulations, the current “DOT Urine Specimen Collection Procedures Guidelines,” and the DOT agency regulations that apply to the employers for whom you perform collections. You must also stay current on changes to these materials.

You must:

  • successfully complete a qualification training program
  • pass a monitored proficiency demonstration

Note: There is no “grandfather” clause or waiver for this requirement. A collector’s qualifications are not location- or collection-site-specific, and eligibility follows the collector anywhere DOT agency-regulated urine specimens are collected.

There is no requirement for qualified collectors to register with or be listed on any federally maintained or federally sponsored registry. However, collectors must maintain documentation, for federal inspection, of successful completion of their training and proficiency demonstration.

Do I need to become “certified” to conduct DOT urine collections or operate a DOT collection site for drug testing?

No. The DOT does not require “certification” of a collector or a collection site to perform DOT urine collections. Only individual collectors, not collection sites, may become qualified.

What should be the content of the qualification training program, and who may conduct it?

Although the DOT has not published a set curriculum for urine collection training, trainers should base their programs on DOT regulations and the latest version of the “Urine Specimen Collection Guidelines.”

The qualification training must include knowledge of Part 40 collection procedures, including:

  • all steps necessary to conduct a proper collection
  • how to complete the Custody and Control Form (CCF)
  • how to handle problem collections, such as shy bladder and tampering
  • understanding of fatal and correctable flaws in collections and paperwork
  • the collector’s responsibility to maintain the integrity, privacy, and security of the process

Anyone may conduct the qualification training. The trainer does not need to be an experienced collector or even a person. Training may be delivered in person, by video, computer programs, the internet, video conferencing, or other equivalent means. There is no minimum time requirement for the training.

Although an exam is not required, it is recommended that training include a method to verify comprehension.

What should the content of the collector proficiency demonstration and who can conduct it?

After completing the training, the collector must perform five error-free mock collections under direct observation by a qualified monitor.

These mock collections must include:

  • 2 routine collections
  • 1 insufficient urine quantity collection
  • 1 temperature out of range collection
  • 1 refusal to sign the CCF or initial the seal collection

Mock collections must be monitored in real time, either in person or via a live video link with direct interaction. A checklist is acceptable if it was part of the training and will be used in real collections.

The monitor must be a qualified collector who:

  • has performed DOT collections for at least one year, or
  • has provided Part 40 training for one year, or
  • has completed a train-the-trainer program

This person must also provide written attestation that all mock collections were error-free.

What kind of documentation must I keep to show that I’m qualified?

Most organizations provide a certificate upon successful course completion. Collectors must be prepared to demonstrate eligibility to DOT inspectors, employers, and service agents, such as C/TPAs.

You should also be ready to provide detailed descriptions of the training and mock collections, such as certificates or signed letters from trainers or observers confirming completion.

Do I have to go through refresher training?

Yes. Qualified collectors must complete refresher training every five years to remain eligible. The content must be the same as or equivalent to the initial training and mock collection. Collectors are expected to stay updated with regulatory changes and not wait for refresher training.

Does DOT offer collector training?

No. The DOT does not offer training, maintain lists of programs or trainers, or certify or endorse any organization’s training.

Collection sites may either conduct their own training or hire an external provider. You can find such services through online searches, industry associations, or training directories.

Once I’m qualified, how do I get business?

Becoming a collector is a business decision. You are responsible for growing your business. Consider transportation trade shows, local directories, or online platforms to find clients regulated by DOT.

Where can I get more information?

Visit the DOT Office of Drug & Alcohol Policy & Compliance (ODAPC) at

Do you need more information?